A multi-level study of recombinant Pichia pastoris in different oxygen conditions

BMC Systems Biology

Table 4 Correlation between microarray ad qRT-PCR data

Target Gene	PIPA ORF	8/21 E array	p-value	8/21 E qPCR	stdev
MDH1	PIPA02244	-1.61	4.90E-03	-2.03	± 0.212
FUM1	PIPA02844	-2.07	2.23E-02	-1.42	± 0.121
YDL124W	PIPA01263	2.58	3.00E-04	1.52	± 0.139
RKI1	PIPA02895	2.44	2.50E-03	1.93	± 0.091
CDC19	PIPA00751	5.24	8.24E-10	3.71	± 0.189
TDH3	PIPA02510	4.34	5.44E-06	2.03	± 0.151
Fab Hc	-	1.65	8.80E-02	1.68	± 0.064
Fab Lc	-	2.07	8.30E-05	1.80	± 0.019
		E/C 8 array	p -value	E/C 8 qPCR	stdev
NCE103	PIPA03864	2.21	2.30E-02	1.71	± 0.087
PFK3	PIPA09969	2.53	1.20E-02	3.21	± 0.128
ERG25	PIPA00945	2.79	3.00E-03	2.21	± 0.093
AQR1	PIPA04502	3.18	3.90E-02	4.12	± 0.188
YCT1	PIPA00376	-9.19	3.90E-04	-5.03	± 0.322
FLR1	PIPA02458	-2.22	4.10E-02	-1.78	± 0.161
Fab Hc	-	2.96	1.75E-01	10.31	± 0.397

Quantitative real-time PCR results compared with microarray data. Standard deviations (stdev) derive from triplicate measurements; all numbers reflect relative gene expression to the reference gene actin (ACT1). PIPA ORF = Pichia pastoris Open Reading Frame (http://www.pichiagenome.org); 8/21 E = pairwise comparison of hypoxic (8) and normoxic (21) conditions in the expressing strain (E); E/C 8 = pairwise comparison of expressing and control (C) strain under hypoxic conditions

ISSN: 1752-0509