Table 2 Estimated rate constants for EGFR phosphorylation/dephosphorylation and adaptor recruitment

Receptor tyrosine phosphorylation rates

   Residue 992

0.055 (nM × s)^-1

   Residue 1068

0.055 (nM × s)^-1

   Residue 1148

0.0063 (nM × s)^-1

   Residue 1173

0.055 (nM × s)^-1

   Multiplier when a receptor is phosphorylated.

3

   Multiplier when a receptor is ligand-bound.

2

Receptor tyrosine dephosphorylation rates

   Residue 992

0.013/s

   Residue 1068

0.013/s

   Residue 1148

0.0014/s

   Residue 1173

0.013/s

   Multiplier when ligand is removed.

4

Adaptor docking rates

   For Grb2

0.0072 (nM × s)^-1

   For Stat5

0.0055 (nM × s)^-1

   For PLCγ1

0.00216 (nM × s)^-1

   For Shc docking to pY1148

0.00936 (nM × s)^-1

   For Shc docking to pY1173

0.0056 (nM × s)^-1

Adaptor dissociation rates

   For Grb2

9.34/s

   For Stat5

11.74/s

   For PLCγ1

21.15/s

   For Shc dissociation from pY1148

13.39/s

   For Shc dissociation from pY1173

13.39/s

K_d of adaptor recruitment to EGFR

   For Grb2

1.3 μM

   For Stat5

2.13 μM

   For PLCγ1

9.8 μM

   For Shc dissociation from pY1148

1.43 μM

   For Shc dissociation from pY1173

2.4 μM