Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production

Table 1 Features of the in silico metabolic model of R.eutropha H16

Features	Number
Genome feature
Genome size (base pairs, bp)	7,416,678
Chromosome 1	4,052,032
Chromosome 2	2,912,490
Megaplasmid pHG1	452,156
No. of open reading frames (ORFs)	6,626 (1,678)^a
Chromosome 1	3,651 (841)
Chromosome 2	2,555 (680)
Megaplasmid pHG1	420 (157)
In silico metabolic model
No. of reactions (redundant) included in the model	1391
No. of biochemical reactions	1162
No. of transport reactions	229
No. of reactions (unique) included in the model^b	1367
No. of metabolites	1171
No. of ORFs assigned in metabolic network	1256
ORF coverage^c (%)	18.96

^a The parenthesis contains the number of the ORFs with unknown function.
^bThe same redundant reactions catalyzed by an isozyme is counted as one.
^cThe number of ORFs incorporated in the genome-scale model divided by the total number of ORFs in the genome of R. eutropha.

ISSN: 1752-0509