Skip to main content

Table 4 Growth phenotypes predicted by the model under a variety of routinely utilized laboratory conditions*

From: iRsp1095: A genome-scale reconstruction of the Rhodobacter sphaeroides metabolic network

 

Light

Dark

  

Electron Acceptor

  

O2a

DMSOa

None

Succinate + NH3

+/+b

+/-

+/-

-/-

Succinate + Glutamate

+/+ b

+/-

+/-

-/-

Lactate + NH3

+/+ b

+/-

+/-

-/-

Glutamate only

+/-

+/-

+/-

-/-

CO2 + H2 + NH3

+/-

-/-

-/-

-/-

CO2 + H2 + N2

+/-

-/-

-/-

-/-

  1. * +/+ Growth and H2 production predicted; +/- Growth but no H2 production predicted; -/- No growth
  2. aOxygen (O2) or DMSO was used as the sole electron acceptors in simulations.
  3. b Succinate and lactate uptake rates were set to 3 mmol/g DW h, while the NH3 and glutamate uptake rates were set to 1 mmol/g DW h, as these are within the rate of experimentally observed uptake rates for these substrates. CO2, H2 and N2 uptake rates were set to 1 mmol/g DW h for photoautotrophic growth simulations.