Figure 1From: Global transcription regulation of RK2 plasmids: a case study in the combined use of dynamical mathematical models and statistical inference for integration of experimental data and hypothesis explorationGenome map of RK2 plasmid. a) RK2 whole genome map, 60,099 bp; KorA, KorB, TrbA, KorC are global regulators. Arrows to the promoters and signs indicate the binding sites of the regulators and a type of regulation (- is repression), oriV and oriT are origins of vegetative replication and transfer, respectively, blac - Tn1 (transportable element), grey - Tra1 and Tra2 (transfer genes), blue - partitioning function, red - the central control operon. b) The central control operon, consisting of korA, incC2/C1, korB, korG/F genes, is regulated by a single promoter (p) and ends at a terminator (t). The operon is negatively and co-operatively auto-regulated by KorA and KorB. c) Binding sites of KorA and KorB on the korAp promoter: KorB binds 40 bp upstream of the transcription start point (tsp), and KorA binds 33 bp downstream from KorB binding site (OB1). KorA binds in -10 region, where RNAP binds.Back to article page