Figure 4

Protein complexes regulated by the miR-141-200c cluster. a, Real-time reverse transcription-PCR of CtBP2 and ZEB1 after transfection of the indicated miRNAs in undifferentiated cancer cells (PANC-1). The expression levels of E-cadherin (of which the transcription is repressed by CtBP/ZEB complex) are included as positive controls. b, Confirmation of the regulation of CtBP2 and ZEB1 by miR-141 and miR-200c on protein levels by immunoblots. c, ZEB1 and CtBP2 knock down by siRNAs, no change in protein levels of the respective complex partner is oberserved. e, Downregulation of CDYL and RCOR3 on protein level when miR-141 or miR-200c was transiently transfected.