Table 2 Model-based predictions and experimental data on net CO2 release, biomass yield and RubisCO flux for photoheterotrophic growth of PNSB on different substrates.
Succinate | Malate | Acetate | Fructose | Propionate | Hydrogen (autotroph) | |
|---|---|---|---|---|---|---|
Net CO2 release | Scenario S1/S2 | Scenario S1/S2 | Scenario S1/S3/S4/S5 | Scenario S1/S2 | Scenario S1/S2 | Scenario S1/S2 |
calculated [mmol CO2/mmol substrate] | 0.72 | 1.19 | 0.13 | 0.38 | -0.28 | -0.47 |
([mmol CO2/C in substrate]) | (0.181) | (0.300) | (0.064) | (0.064) | (-0.094) | (∞) |
measured [mmol CO2/mmol substrate] | 0.75 [37] | 1.15 [37] | 0.11 [10] | -0.23 [37] | ||
0.70 [36] | 1.22 [36] | 0.25 [37] | ||||
0.17 [36] | ||||||
Biomass yield Y X/S | Scenario S1/S2 | Scenario S1/S2 | Scenario S1/S3/S4/S5 | Scenario S1/S2 | Scenario S1/S2 | Scenario S1/S2 |
calculated [gDCW/mmol substrate] | 0.072 | 0.062 | 0.041 | 0.124 | 0.072 | 0.010 |
calculated [gDCW/g substrate] | 0.610 | 0.463 | 0.683 | 0.688 | 0.973 | 5.000 |
measured [gDCW/g substrate] | 0.476 [52] | 0.660 [44] | ||||
0.650[10] | ||||||
RubisCO flux | Scenario S2 | Scenario S2 | Scenario S3/S4/S5 | Scenario S2 | Scenario S2 | Scenario S2 |
Calculated minimal RubisCO flux [mmol/mmol substrate] | 0.73 | 0.20 | S3: 0.22 | 0.40 | 0.73 | 0.53 |
S4: 0.22 | ||||||
S5: 0 | ||||||
[mmol/mmol C in substrate] | (0.184) | (0.05) | S3: 0.11 | (0.067) | (0.245) | (∞) |
S4: 0.11 | ||||||
S5: 0 | ||||||
Measured [mmol/mmol substrate] | S3: 0.29[10] |