Compartmentation of glycogen metabolism revealed from 13C isotopologue distributions

Marin de Mas, Igor; Selivanov, Vitaly A; Marin, Silvia; Roca, Josep; Orešič, Matej; Agius, Loranne; Cascante, Marta

doi:10.1186/1752-0509-5-175

BMC Systems Biology

Table 2 Isotopologue distribution produced by isolated hepatocytes in the presence of glucose and lactate.

From: Compartmentation of glycogen metabolism revealed from ¹³C isotopologue distributions

	Experiment 1	Simulations		Experiment 2	Simulations
	label in glucose	B	A	label in lactate	B	A
glucose:		χ ² = 7.21	5.72		χ ² = 5.52	0.576
m0	0.532 ± 0.0098	0.514	0.514	0.979 ± 0.01	0.976	0.984
m1	0.00846 ± 0.0022	0.0114	0.00918	0.0063 ± 0.0055	0.00484	0.00354
m2	0.459 ± 0.0103	0.473	0.475	0.0055 ± 0.0064	0.00891	0.00469
m3	-- --	--	--	0.0064 ± 0.0016	0.00996	0.00628
[mM]	20.6 ± 2.91	21	21.1	20.9 ± 2.22	20.8	20.9
glycogen:		χ ² = 6.46	27.3		χ ² = 2.22	5.73
m0	0.681 ± 0.032	0.683	0.57	0.909 ± 0.026	0.907	0.9
m1	0.0119 ± 0.031	0.00767	0.0131	0.017 ± 0.0066	0.0166	0.0225
m2	0.302 ± 0.031	0.308	0.409	0.038 ± 0.01	0.0313	0.0298
m3	0.0017 ± 0.001	0.000136	0.00208	0.0273 ± 0.0071	0.0363	0.04
m4	0.0032 ± 0.0016	0.000593	0.00565	0.0036 ± 0.0016	0.00329	0.00317
m5	-- --	--	--	0.003 ± 0.014	0.00335	0.00271
mg/mL	0.263 ± 0.084	0.256	0.196	0.262 ± 0.0691	0.256	0.196
glgn14:		χ ² = 5.31	19.2		χ ² = 2.23	4.59
m0	0.678 ± 0.032	0.692	0.588	0.93 ± 0.024	0.924	0.921
m1	0.016 ± 0.0046	0.00561	0.0112	0.033 ± 0.009	0.0343	0.0472
m2	0.3 ± 0.032	0.302	0.399	0.019 ± 0.0079	0.0178	0.0128
m3	-- --	--	--	0.014 ± 0.005	0.0209	0.0163
m4	-- --	--	--	0.004 ± 0.003	0.00267	0.0264
glgn36:		χ ² = 1.48	14.2		χ ² = 1.97	4.48
m0	0.98 ± 0.0101	0.987	0.968	0.924 ± 0.024	0.923	0.911
m1	0.00408 ± 0.0018	0.0051	0.0101	0.0265 ± 0.007	0.0332	0.0348
m2	0.0139 ± 0.0078	0.00766	0.216	0.027 ± 0.0081	0.0188	0.0219
m3	-- --	--	--	0.021 ± 0.0067	0.0221	0.0292
lactate:		χ ² = 2.06	2.73		χ ² = 1.62	9.76
m0	0.974 ± 0.026	0.991	0.985	0.636 ± 0.017	0.621	0.608
m1	0.0026 ± 0.0019	0.000974	0.00135	0.0166 ± 0.0025	0.0167	0.0172
m2	0.0094 ± 0.0037	0.00773	0.0141	0.0318 ± 0.0035	0.0302	0.0245
m3	0.00136 ± 0.023	0.00000227	0.0000436	0.316 ± 0.0213	0.332	0.35
[mM]	6.18 ± 0.75	6.8	6.73	3.18 ± 0.43	6.29	6.22
Σχ ²		22.52	69.15		13.56	25.136

Before incubation the medium contained either 50% of [1,2-13C2]D-glucose and unlabeled lactate (experiment 1) or 50% uniformly 13C-labeled lactate and unlabeled glucose (experiment 2). The measurements are presented as mean ± standard deviation. The data were fit by two models (A and B). The conditions of incubation and measurements, and data fitting are described in Methods. The difference between the best fit and experimental data (χ2, see Methods) are shown for each metabolite and summarized for the whole set of data.

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ISSN: 1752-0509

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