Figure 6

Time-resolved VASP phosphorylation upon administration of cAMP elevating drug doses. Ratios of phosphorylated VASP sites (Ser157, Ser239) to the total amount of VASP in platelets are measured after administration of doses of AC stimulator Iloprost (2 nM, 5 nM, 10 nM) at distinct time points (black circles ± calculated SD). The experimentally measured cAMP time course (top panel of each row), marked with black stars serves as model input. Phosphosite Ser157 is phosphorylated prior to Ser239 by PKA as indicated by data and model (red trajectories).