Figure 3

ESAT-6 downregulated module and novel virulence factors. Genes significantly downregulated by ranalexin (RanaDown) are shown in pink, other genes in yellow. Two significant network modules are shown (p ≤ 0.025, p ≤ 1.9E^-4). Names (e.g. esxA) are given for genes that have been characterised, and these all represent ESAT-6 system components; otherwise locus identifiers are given (e.g. SAR0294). Network edges are shown as blue lines. Novel virulence roles were inferred for the SAR0292-0294 module and the seven uncharacterised genes in the ESAT-6 module (see main text). These network-based predictions are supported by several further lines of evidence, including predicted cellular localisation, domain matching and operon structure. This figure was generated in Cytoscape [104].