Table 2 Definition of the species that participate in the tet operon reaction network.
Species | Definition |
|---|---|
TcEx | External Tc |
Tc | Intracellular Tc |
TetR | Tc repressor protein |
TetR2 | Tc repressor protein (dimer) |
TetA | Tc transport protein |
tetO1 | Operator site 1 |
tetO2 | Operator site 2 |
nsDNA | Non-specific DNA sites |
tetP R 1 | Promoter 1 of tetR gene |
tetP R 2 | Promoter 2 of tetR gene |
tetP A | Promoter of tetA gene |
RNAp | RNA polymerase |
Complex | Free operator and promoter sites |
mRNA(tetA) | tetA gene mRNA |
mRNA(tetR) | tetR gene mRNA |
Rib | Ribosome |
Rib:mRNA(tetR) | Ribosome bound to mRNA(tetR) |
Rib:mRNA(tetA) | Ribosome bound to mRNA(tetA) |
TetR 2:tetO1 | TetR2 bound to tetO1 |
TetR 2:tetO2 | TetR2 bound to tetO2 |
TetR 2:nsDNA | TetR2 bound to nsDNA |
TetR 2:Tc | TetR2 bound to one Tc molecule |
TetR 2:Tc 2 | TetR2 bound to two Tc molecules |
TetR 2:tetO1:Tc | TetR2 bound to tetO1 and one Tc molecule |
TetR 2:tetO1:Tc 2 | TetR2 bound to tetO1 and two Tc molecules |
TetR 2:tetO2:Tc | TetR2 bound to tetO2 and one Tc molecule |
TetR 2:tetO2:Tc 2 | TetR2 bound to tetO2 and two Tc molecules |
RNAp:[tetPR 1]:tetPR 2:tetP A :tetO1:tetO2 | Closed complex of RNAp bound to tetPR 1 |
RNAp*:[tetPR 1]:tetPR 2:tetP A :tetO1:tetO2 | Open complex of RNAp bound to tetPR 1 |
RNAp:tetPR 1:[tetPR 2]:tetPA:tetO1:tetO2 | Closed complex of RNAp bound to tetPR 2 |
RNAp*:tetPR 1:[tetPR 2]:tetP A :tetO1:tetO2 | Open complex of RNAp bound to tetPR 2 |
RNAp:[tetPR 2]:tetO1:TetR 2:tetO2 | Closed complex of RNAp bound to tetPR 2 |
RNAp*:[tetPR 2]:tetO1:tetR 2:tetO2 | Open complex of RNAp bound to tetPR 2 |
RNAp:tetPR 1:tetPR 2:[tetP A ]:tetO1:tetO2 | Closed complex of RNAp bound to tetP A |
RNAp*:tetPR 1:tetPR 2:[tetP A ]:tetO1:tetO2 | Open complex of RNAp bound to tetP A |
Rib*:mRNA(tetA) | Rib bound to the first codon of the mRNA(tetA) |
Rib*:mRNA(tetR) | Rib bound to the first codon of the mRNA(tetR) |
RNAp*:DNA(P A ) | RNAp bound to tetA gene |
RNAp*:DNA(PR 1) | RNAp bound to tetR gene starting transcription from tetPR 1 |
RNAp*:DNA(PR 2) | RNAp bound to tetR gene starting transcription from tetPR 2 |