Figure 7
From: Modeling of human factor Va inactivation by activated protein C
Effect of Prothrombin on the APC Inactivation of FVa. Empirical: FVa (20 nM) was pre-incubated with prothrombin (1.4 μM) at 37°C. The reaction was initiated with 2.0 nM (red circles) or 20.0 nM (blue circles) APC. A representative Western blot is shown in the Panel A inset. Data are normalized densitometric values for the following fragments: FVaHC (Panel A), FVa1-506 (B), FVa307-679/709 (C) and FVa307-506 (D). Data points are the averages ± S.D. of either 4 or 3 experiments for inactivation studies conducted with either 2.0 or 20.0 nM APC, respectively. Mathematical: Simulated time courses (2.0 nM APC – red lines; 20.0 nM APC – blue lines) were generated under two modeling conditions: 1) The assumption of unlimited binding sites for all species and a KD of 700 nM for FVaPT complex (dash and dotted lines) [39]; and 2) The assumption of limited binding sites, thus reducing the effective APC concentration, with hypothetical KD values for the FVaPT complex of 0 nM (dotted line), 500 nM (dashed line), or 700 nM (solid line) are shown.