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Table 4 SrbA binding sites

From: Regulatory interactions for iron homeostasis in Aspergillus fumigatus inferred by a Systems Biology approach

Gene

Position

Strand

Sequence

k a (M-1s-1)

k d (s-1)

K D (nM)

srbA

-735 to -726

Sense

ATCATACGAT

1.48 ± 0.05 * 107

9.27 ± 0.29 * 10-3

0.63 ± 0.04

srbA*

-735 to -726

Sense

ATATAACATA

5.92 ± 0.08 * 105

2.29 ± 0.01* 10-1

386 ± 7.3

hapX

-774 to -783

Antisense

ATCCTCCCAT

5.68 ± 0.07 * 105

1.61 ± 0.01* 10-1

282 ± 5.1

hapX

-1340 to -1331

Sense

ATCAGATGAT

3.10 ± 0.01 * 107

1.44 ± 0.01 * 10-1

4.64 ± 0.03

hemA

-527 to -518

Sense

ATCGGATCAT

1.41 ± 0.07 * 107

5.88 ± 0.28 * 10-2

4.18 ± 0.41

hemA

-338 to -347

Antisense

ATCGCCTCAT

1.03 ± 0.01 * 106

3.66 ± 0.02 * 10-2

35.7 ± 0.52

  1. The binding of SrbA to five predicted binding sites was analysed. Results indicate three high-affinity binding sites in promoter regions of hapX, hemA and srbA. The asterix denotes a mutated binding site. Mutated nucleotides are underlined. k a = association rate constant, k d = dissociation rate constant, K D = k d /k a equilibrium dissociation constant.