Figure 4

A. The transfection of mir-29a mimics into HCT-116 cells down-regulates KLF4 mRNA expression (Left, QRT-PCR) and protein expression (Right, Western blot). B. The transfection of mir-29a inhibitor into HCT-116 cells up-regulates KLF4 mRNA expression (Left, QRT-PCR) and protein expression (Right, Western blot). The experiments were independently repeated three times and GADPH served as control. ***, P < 0.001.