Mapping global effects of the anti-sigma factor MucA in Pseudomonas fluorescens SBW25 through genome-scale metabolic modeling

Table 2 Physiological characteristics of the strains and conditions studied

Carbon source	Strain	OD ₆₆₀	C source Uptake [mmolC/ gDW h]	Alginate production, deacetylated [mmolC/gDW h]	Acetate (acetyl from alginate) [mmolC/gDW h]	CO ₂ excretion [mmolC/ gDW h]	C in biomass [mmolC/ gDW h]	C balance [%]
Fructose	Wild-type	7,8	9,4	-	-	7,5	1,51	95,7
Fructose	mucA	7,0	18,3	12,3	0,77	4,6	1,51	104,4
Fructose	ΔalgC	8,1	9,1	-	-	7,4	1,51	97,6
Fructose	mucA ΔalgC	8,4	5,8	-	-	4,3	1,51	99,3
Fructose	mucA TTalgD	9,2	5,6	-	-	3,9	1,51	96,9
Glycerol	SBW25	9,2	8,3	-	-	6,4	1,51	96,3
Glycerol	mucA	7,4	17,5	10,6	1,22	3,6	1,51	96,8
Glycerol	mucA TTalgD	9,9	6,9	-	-	5,0	1,51	95,4

Measured and calculated physiological parameters for chemostat cultivations (D = 0.04) of P. fluorescens SBW25 (wild-type) and derived mutant strains. The value for mmolC/gDW h in biomass is calculated from the specific growth rate used in the experiments (μ = 0.04 hr^-1) and the biomass stoichiometric composition assumed in the genome scale model. The % value for carbon balance represents the sum of carbon accounted for in alginate, CO₂ and biomass as a percentage of the carbon taken up by the cells.

ISSN: 1752-0509