Figure 2From: The last generation of bacterial growth in limiting nutrientUnder nutrient limitation decline in growth rate is well described by Monod equation. (a) Final OD correlates linearly with initial substrate (NH4Cl) level: ODf(s) = a*s + b, with a = 0.066 ± 0.003 and b = 0.006 ± 0.001. This relation only saturates at high (more than 2 mM) initial nitrogen level (inset). The intersect of the linear graph is not zero probably due to low levels of nitrogen which were transferred to the media from the over-night culture together with the bacterial inoculum. (b) Growth rate as a function of substrate level, calculated using the linear relation between OD and substrate levels in (a). Line: fit to Monod law, with Ks = 2.6 ± 0.4 μM. The 0.31 mM curve deviates from the Monod fit more than other curves for unknown reasons.Back to article page