Levels of pro-apoptotic regulator Bad and anti-apoptotic regulator Bcl-xL determine the type of the apoptotic logic gate

Table 3 Macro-parameters governing steady-state solution levels

Macro-parameter definition	Value	Unit	Description
$m_{1} : = \frac{s_{1} s_{4}}{d_{1} d_{2}}$	2 × 10 ⁴	mlcl	${Bax}_{tot}^{min}$
$m_{2} : = (s_{1} + s_{2} \frac{{(p 5 3_{killer}^{max})}^{2}}{M^{2} + {(p 5 3_{killer}^{max})}^{2}}) \frac{s_{4}}{d_{1} d_{2}}$	6.8 × 10 ⁴	mlcl	${Bax}_{tot}^{max}$
$m_{3} : = \frac{b_{1}}{u_{1}}$	0.3	mlcl⁻¹	Bax–Bcl-x_L affinity
$m_{4} : = \frac{b_{2}}{u_{2}}$	30	mlcl⁻¹	Bcl-x_L–Bad_u affinity
$m_{5} : = \frac{b_{3}}{u_{3}}$	30	mlcl⁻¹	Bad_p–Scaffold_14-3-3 affinity
$m_{6} : = \frac{p_{1} Ak t_{tot}}{q_{1} + p_{1} Ak t_{tot}}$	$\frac{2}{3}$	—	max. fraction of phosphorylated Bad
$m_{7} : = \frac{d_{3} S - a_{2} S^{2} (\frac{s_{3}}{d_{3}} - S)}{a_{1} (\frac{s_{3}}{d_{3}} - S)}$ ,	≅ 5000	mlcl	Bax_bif (at Casp≅ 1000)
whereS=Casp+Procasp

ISSN: 1752-0509