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Figure 4 | BMC Systems Biology

Figure 4

From: Robust network structure of the Sln1-Ypd1-Ssk1 three-component phospho-relay prevents unintended activation of the HOG MAPK pathway in Saccharomyces cerevisiae

Figure 4

Moderate overexpression of phospho-relay components causes mild activation of HOG pathway transcriptional targets on long timescales. STL1 is a gene induced in response to active Hog1. We replaced one copy of the STL1 gene with yEVenus in homozygous GEV strains to create a P S T L1-yEVenus transcriptional reporter of HOG pathway activity. We used flow cytometry to analyze yEVenus expression in cells at the start of the experiment and at 2 hours and 19 hours after induction with 10 μM β-estradiol. Pbs2 and Ssk22 overexpression caused the strongest activation of the HOG pathway transcriptional reporter. Cell counts are plotted against the natural log of their fluorescence signal [a.u.].

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BMC Systems Biology

ISSN: 1752-0509

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