Table 6 Principal experimental methods for GE quantification
Method | Pros | Cons |
|---|---|---|
Northern Blotting | -Inexpensive | -low throughput |
-detecting transcript size | -semiquantitative | |
-RNAase contamination | ||
RT-PCR | -high sensitivity | -high variability |
-high sequence specific | -normalizaton methods | |
Microarray | -measurement of the activity of thousands of genes at once | -high cost |
-rapid | -analysis of Big data | |
-don't require large-scale DNA sequencing | -high Background noise | |
Sanger sequencing technology | -low Background noise | -only a portion of the transcript |
-isoforms are generally indistinguishable from each other | ||
-Low throughput | ||
RNA-seq | -measurement of the activity of thousands of genes at once | -High cost |
-require low amount of RNA | -Analysis of Big data | |
-high reproducibility | ||
-Low Background noise |