Fig. 2

qPCR validation and NPC proliferation assay. a qPCR analysis of six DEGs using neuronal RNAs from 4–5 controls and 4–5 patients. Relative expression values were determined and pooled by phenotype. Error bars show standard error of the mean; Two-tailed t-test p-value <0.01 (**) and <0.05 (*) are indicated. b Proliferation rates of NPCs derived from control (n = 4) and 22q.11.2 (n = 4) iPSCs. An equal number of cells were plated for each sample (10,000) on day 0. Fold changes were calculated by normalizing the day 1 controls to 1.00, and the control and patient samples were pooled. Statistical significance was determined using a two-tailed Student’s t-test. The asterisk denotes statistically significant difference in proliferation at p < 0.05