Fig. 1

Flowchart of the Agent-Based Model. The initial 100 cells are set up based on the flow cytometry results for the cell line MDA-MB-231 (MB231). These initial 100 cells were placed in a cubic grid 100x100x80 voxels, each voxel 20x20x20 microns, one cell per voxel. The cells become quiescent if they have no adjacent space. The cells migrate based on their CCR5 expression levels. Stem cells can divide symmetrically or asymmetrically, whereas progenitor cells divide only symmetrically. If a progenitor cell has reached its division limit, it becomes senescent. Each day a senescent cell has a 10% chance of dying