Fig. 4

Effects of TKI concentrations on paclitaxel efflux influenced by P-gp level. Increasing doses of TKIs promote the accumulation of intracellular paclitaxel. The initial concentrations of extracellular paclitaxel and intracellular ATP were set to 3 μM and 3 mM, respectively. Higher doses of dasatinib (blue curve) were required to prevent paclitaxel efflux than imatinib and nilotinib (green and red curves, respectively). The P-gp levels were 0.1 μM (top left), 1 μM (top right), 10 μM (bottom left), and 100 μM (bottom right)