Integrated analysis, transcriptome-lipidome, reveals the effects of INO- level (INO2 and INO4) on lipid metabolism in yeast

Table 2 Physiological parameters of reference and mutants in chemostat cultivation

Strains	µ_max¹	Left Glc (g/L)	RQ	Y_SX²	Y_SE³	Y_SG⁴	Y_SA⁵	Y_SS⁶	Y_SC⁷
RefC	0.384 ± 0.001	0.015 ± 0.000	0.817 ± 0.007	0.539 ± 0.010	n.d.	0.019 ± 0.001	n.d.	n.d.	0.124 ± 0.003
Opi1CT	0.343 ± 0.001	0.009 ± 0.001	1.058 ± 0.020	0.557 ± 0.002	0.004 ± 0.000	0.019 ± 0.000	0.003 ± 0.000	n.d.	0.208 ± 0.004
Opi1CB	0.343 ± 0.001	0.009 ± 0.001	0.819 ± 0.003	0.550 ± 0.001	n.d.	0.019 ± 0.000	n.d.	n.d.	0.106 ± 0.003
Ino2C	0.340 ± 0.000	0.018 ± 0.001	0.805 ± 0.011	0.597 ± 0.020	n.d.	0.018 ± 0.001	0.003 ± 0.000	n.d.	0.129 ± 0.006
Ino4C	0.369 ± 0.001	0.010 ± 0.000	0.837 ± 0.009	0.571 ± 0.007	n.d.	0.018 ± 0.002	n.d.	n.d.	0.135 ± 0.002
Ino24C	0.436 ± 0.000	0.039 ± 0.001	0.889 ± 0.013	0.362 ± 0.028	0.098 ± 0.011	0.022 ± 0.003	0.019 ± 0.000	0.002 ± 0.000	0.101 ± 0.002
RefN	0.486 ± 0.000	20.387 ± 1.667	1.286 ± 0.008	0.128 ± 0.004	0.485 ± 0.048	0.008 ± 0.000	0.007 ± 0.001	0.002 ± 0.000	0.124 ± 0.004
Opi1N	0.499 ± 0.001	15.521 ± 1.809	1.260 ± 0.001	0.096 ± 0.003	0.316 ± 0.017	0.005 ± 0.000	0.005 ± 0.000	n.d.	0.075 ± 0.017
Ino2N	0.495 ± 0.001	28.286 ± 0.877	1.159 ± 0.008	0.168 ± 0.002	0.305 ± 0.015	0.009 ± 0.000	0.006 ± 0.001	n.d.	0.102 ± 0.005
Ino4N	0.494 ± 0.001	28.837 ± 0.427	1.261 ± 0.017	0.164 ± 0.003	0.337 ± 0.010	0.008 ± 0.000	0.006 ± 0.000	n.d.	0.103 ± 0.001
Ino24N	0.410 ± 0.000	35.944 ± 1.867	1.269 ± 0.010	0.058 ± 0.008	0.370 ± 0.026	0.017 ± 0.001	0.038 ± 0.001	n.d.	0.076 ± 0.005

Note: All values are average ± SD from three biological replicates.
¹ maximum specific growth rate on glucose during batch cultivation (with 10 g/L and 60 g/L initial glucose for C-limited and N-limited conditions, respectively)
² Biomass yield on glucose in chemostat cultures (g biomass formed/g glucose consumed)
³ Ethanol yield on glucose in chemostat cultures (g ethanol formed/g glucose consumed)
⁴ Glycerol yield on glucose in chemostat cultures (g glycerol formed/g glucose consumed)
⁵ Acetate yield on glucose in chemostat cultures (g acetate formed/g glucose consumed)
⁶ Succinate yield on glucose in chemostat cultures (g succinate formed/g glucose consumed)
⁷ CO₂ yield on glucose in chemostat cultures (mg CO2 formed/g glucose consumed)

ISSN: 1752-0509