Spot intensities of culture images are used for kinetic analysis of proliferation. An overnight culture was serially diluted, by 2-fold, across a 96- well plate. (a) Raw image intensities are plotted versus time for a representative culture at each dilution. (b) The images of spot cultures for each data point in panel (a) are shown. (c) After fitting the data to the logistic equation, TMR, which is the time at which the overall population growth rate is maximal, was calculated for each curve and is plotted versus time. The difference between values of TMR for each curve reflects the doubling time, since cultures were created by serial two-fold dilution. (d) The spot intensities were normalized by spot area, and curves were shifted on the time axis by the difference between TMR of the culture and TMR of the 256x-diluted culture.