Figure 3

A common set of genes is misexpressed in sticky and dFmr1 mutants. (A) Three biological replicates of sticky total RNA and three biological replicates of dFmr1 mutants were analyzed by NimbleGen gene expression arrays. These data were compared to expression data from two biological replicates of OrR and two biological replicates of w¹¹¹⁸ wild-type control RNA. Expression data from the two controls were combined in order to better account for differences in genetic background. (B-C) Venn diagrams showing the number of genes with significantly altered expression levels in sticky and dFmr1 mutants. (B) With a significance threshold of q<.001, we found 249 misexpressed genes and 1,044 misexpressed genes in sticky and dFmr1 mutants, respectively. There were 40 genes differentially expressed in both mutant strains (p = 2.37e-6) and 14,322 genes with no significant change in expression. (C) With a threshold of q<.01, we found 771 misexpressed genes and 2,894 misexpressed genes in sticky and dFmr1 mutants, respectively. There were 246 genes differentially expressed in both mutants (p = 4.27e-14) and 11,999 genes with no significant change in expression. (D) Of the genes that were misexpressed in either mutant the vast majority of them were overexpressed.