Table 5 Kinetic parameters of VEGF in tumor (breast cancer)

VEGF binding to VEGFR1

k_on

3 10⁷

M^-1 s^-1

5.8 10^-2

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-3

s^-1

K_d

33

pM

1.7 10^-2

pmol/cm³ tissue

VEGF binding to VEGFR2

k_on

10⁷

M^-1 s^-1

1.9 10^-2

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-3

s^-1

K_d

100

pM

5.2 10^-2

pmol/cm³ tissue

VEGF165 binding to NRP1

k_on

3.2 10⁶

M^-1 s^-1

6.1 10^-3

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-3

s^-1

K_d

312

pM

1.6 10^-1

pmol/cm³ tissue

VEGF165 binding to GAGs

k_on

4.2 10⁵

M^-1 s^-1

8.1 10^-3

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-2

s^-1

K_d

24

nM

12.5

pmol/cm³ tissue

Coupling of NRP1 & VEGFR2

k_cV165R2,N1

3.1 10¹³

(mol/cm²)^-1 s^-1

2.9 10¹¹

(pmol/cm³ tissue)^-1 s^-1

k_offV165R2,N1

10^-3

s^-1

k_cV165N1,R2

10¹⁴

(mol/cm²)^-1 s^-1

9.5 10¹¹

(pmol/cm³ tissue)^-1 s^-1

k_offV165N1,R2

10^-3

s^-1

VEGFR1 coupling to NRP1

k_cR1,N1

10¹⁴

(mol/cm²)^-1 s^-1

9.5 10¹¹

(pmol/cm³ tissue)^-1 s^-1

k_dissocR1,N1

10^-2

s^-1

VEGFR internalization

k_int,R

2.8 10^-4

s^-1

k_int,C

2.8 10^-4

s^-1