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Table 4 Mutant phenotyping experiments: growth media and experimental results for genes included in iAbaylyiv3

From: Iterative reconstruction of a global metabolic model of Acinetobacter baylyi ADP1 using high-throughput growth phenotype and gene essentiality data

Source of1 Essentiality Specific metabolic pathways a priori involved
Carbon nitrogen E D  
acetate ammonia 5 431 Glyoxylate shunt
L-asparagine ammonia 3 445 Asparagine and aspartate degradation
D-2,3-butanediol ammonia 10 433 Butanediol to acetoin to acetyl-coa degradation, glyoxylate shunt
D-glucarate ammonia 5 413 Glucarate to 2-oxoglutarate degradation
β-D-glucose ammonia 7 432 Entner-Doudoroff pathway
L-lactate ammonia 2 445 Lactate dehydrogenase
quinate ammonia 8 436 Quinate to protocatechuate to acetyl-coa and succinyl-coa degradation
succinate urea 3 442 Urease
  1. 1 Italic text indicates the changed carbon or nitrogen source with respect to the medium used for mutant construction (succinate and ammonia).
  2. E: number of conditionally essential genes
  3. D: number of dispensable genes