Figure 5

Analysis of the effects of knockdowns on G1/S transition in trastuzumab resistant HCC1954 cells. A. Phosphorylation state of pRB (output of the network) after 12 hour EGF stimulation (input of the network). Box plots show the quantitative phosphorylation of pRB protein for the knockdown of each network protein compared to MOCK (only transfection reagent, no siRNA). B. G1/S ratio after 18 hours of EGF stimulation for corresponding knockdowns compared to MOCK (solid line) and p21 knockdown (dotted line) using 7-AAD DNA staining.