Investigating the robustness of the classical enzyme kinetic equations in small intracellular compartments

BMC Systems Biology

Table 3 Maximum Percentage error in reaction velocity from prediction of the MM equation for Model III.

D/nm	K_M= 10 μM	100 μM	1000 μM	M (burst size)
50	67.8 [76.8]	67.8 [76.5]	67.8	1
100	20.8 [26.4]	20.6 [26.1]	20.6	1
200	2.8	2.7	2.7	1
50	1001.8	234.9 [169.4]	86	50
100	343.7 [345.5]	73.4 [75.2]	26.2 [31.5]	50
200	71.4	11.3 [11.5]	3.6	50

The total number of enzyme molecules per compartment is ten in all cases. Values in bold and in square brackets are those estimated by simulation; the italic values are obtained from the theoretical expressions, Eqs. (7) and (12). The parameters are: k₀ = 10⁹ s^-1 M^-1, k₁ = k₃ = 1000 s^-1, k₄⁰ = 10⁷ s^-1 M^-1, and [I] = 10 [E_T].

ISSN: 1752-0509