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Table 3 Maximum Percentage error in reaction velocity from prediction of the MM equation for Model III.

From: Investigating the robustness of the classical enzyme kinetic equations in small intracellular compartments

D/nm K M = 10 μM 100 μM 1000 μM M (burst size)
50 67.8 [76.8] 67.8 [76.5] 67.8 1
100 20.8 [26.4] 20.6 [26.1] 20.6 1
200 2.8 2.7 2.7 1
50 1001.8 234.9 [169.4] 86 50
100 343.7 [345.5] 73.4 [75.2] 26.2 [31.5] 50
200 71.4 11.3 [11.5] 3.6 50
  1. The total number of enzyme molecules per compartment is ten in all cases. Values in bold and in square brackets are those estimated by simulation; the italic values are obtained from the theoretical expressions, Eqs. (7) and (12). The parameters are: k0 = 109 s-1 M-1, k1 = k3 = 1000 s-1, k40 = 107 s-1 M-1, and [I] = 10 [ET].