A model invalidation-based approach for elucidating biological signalling pathways, applied to the chemotaxis pathway in R. sphaeroides

Table 2 Strains and plasmids

Strain/Plasmid	Characteristics	Source
R. sphaeroides WS8N	Spontaneous nalidixic acid resistant mutant of wild type WS8	[34]
R. sphaeroides JPA421	WS8N with the cheY₄ gene deleted by genomic replacement	[35]
E. coli S17-1 λ pir	A strain capable of mobilising pAE and pAY4 into R. sphaeroides, Sm^R	[36]
Plasmids
pIND4	Over expression vector with a lac inducible promoter, capable of replication in R. sphaeroides	[25]
pIND4-Y4	pAE containing cheY₄	[29]

ISSN: 1752-0509