Scatterplots illustrate the effect of Pmar1-perturbations. Scatterplots of HesC (left) and Alx1 (right) mRNA abundance. Simulated abundances at timepoint t = 25 hpf for all 800 parameter sets are plotted. Red dots represent simulation results under unperturbed (x-axis) and Pmar1-KD (y-axis) conditions. Green dots correspond to simulation results under unperturbed (x-axis) and Pmar1-MOE (y-axis) conditions. In these examples, the different perturbations are clearly distinguishable. Due to the double-negative regulation (Pmar1 inhibits HesC inhibits Alx1), the perturbations in Pmar1 cause converse effects in the expression of HesC and Alx1.