Data calibration. (A) Scatter plot of flow cytometry forward scatter versus side scatter. Excluded data points are shown in light grey. Dashed lines indicate chosen cut for data exclusion. (B) X2curves for the quantile-quantile plot versus the cut position for the flow cytometry data for cell volume (dashed line) and fluorescence intensity (solid line). (C) Quantile-quantile plots for cell volumes (left) and fluorescence intensities (right) used for calibration. Cell populations treated with 10 ng/ml and 250 ng/ml doxycycline are both included. Flow cytometry data are raw data, microscopy data have been transformed to represent the same parameters as flow cytometry data. The number of quantiles corresponds to the number of microscopy data points.