Molecular characterization of bzip1-1. (A) Relative position of T-DNA insertion in bZIP1 gene and primers used for the real-time PCR (FP: forward primer; RP: reverse primer). (B) Comparative PCR of the flanking region T-DNA insertion site. (C) Expression of bZIP1 mRNAs in WT siblings and bzip1-1 plants. Transcript levels are determined by real-time PCR and are shown as relative to expression of Clathrin gene. Values are the mean ± SE from three biological replicates.