Table 1 Summary of the experimental evidence
INTERACTIONS | EXPERIMENTAL EVIDENCE | REFERENCE |
|---|---|---|
SHR →SCR | The expression of SCR is reduced in shr mutants. ChIP-QRTPCR experiments show that SHR directly binds in vivo to the regulatory sequences of SCR and positively regulates its transcription. | |
SCR →SCR | In the scr mutant background promoter activity of SCR is absent in the QC and CEI. A ChIP-PCR assay confirmed that SCR directly binds to its own promoter and directs its own expression. | |
JKD →SCR | SCR mRNA expression as probed with a reporter lines is lost in the QC and CEI cells in jkd mutants from the early heart stage onward. | [17] |
MGP--|SCR | The double mutant jkd mgp rescues the expression of SCR in the QC and CEI, which is lost in the jkd single mutant. | [17] |
SHR →MGP | The expression of MGP is severely reduced in the shr background. Experimental data using various approaches have suggested that MGP is a direct target of SHR. This result was later confirmed by ChIP-PCR. | |
SCR →MGP | SCR directly binds to the MGP promoter, and MGP expression is reduced in the scr mutant background. | |
SHR →JKD | The post-embryonic expression of JKD is reduced in shr mutant roots. | [17] |
SCR →JKD | The post-embryonic expression of JKD is reduced in scr mutant roots. | [17] |
SCR →WOX5 | WOX5 is not expressed in scr mutants. | [24] |
SHR →WOX5 | WOX5 expression is reduced in shr mutants. | [24] |
ARF(MP) →WOX5 | WOX5 expression is rarely detected in mp or bdl mutants. | [24] |
ARF→PLT | PLT1 mRNA region of expression is reduced in multiple mutants of PIN genes, and it is overexpressed under ectopic auxin addition. PLT1 & 2 mRNAs are absent in the majority of mp embryos and even more so in mp nph4 double mutant embryos. | |
Aux/IAA--|ARF | Overexpression of Aux/IAA genes represses the expression of DR5 both in the presence and absence of auxin. Domains III & IV of Aux/IAA genes interact with domains III & IV of ARF stabilizing the dimerization that represses ARF transcriptional activity. | |
Auxin--| Aux/IAA | Auxin application destabilizes Aux/IAA proteins. Aux/IAA proteins are targets of ubiquitin-mediated auxin-dependent degradation. | [reviewed in [18]] |
CLE40 --| WOX5 | Wild type root treated with CLE40p show a reduction of WOX5 expression, whereas in cle40 loss of function plants WOX5 is overexpressed. | [25] |