Edelfosine-induced metabolic changes in cancer cells that precede the overproduction of reactive oxygen species and apoptosis

Table 2 Isotopomer distribution in lactate secreted into the medium and RNA ribose.

Lactate:	control	sd	fit	0.5 μg/mL	sd	fit	1 μg/mL	sd	fit
m0	0.7900	0.003	0.7900	0.7860	0.001	0.7860	0.7910	0.003	0.7920
m1	0.0097	0.001	0.0096	0.0099	0.000	0.0099	0.0098	0.001	0.0098
m2	0.1990	0.003	0.1990	0.2030	0.001	0.2030	0.1980	0.003	0.1970
m3	0.0013	0.001	0.0013	0.0013	0.001	0.0013	0.0010	0.002	0.0011
Rib-5-P
m0	0.5480	0.011	0.5480	0.5570	0.003	0.5570	0.5790	0.011	0.5790
m1	0.2310	0.004	0.2310	0.2210	0.007	0.2250	0.2130	0.004	0.2110
m2	0.1490	0.005	0.1490	0.1410	0.003	0.1430	0.1340	0.005	0.1390
m3	0.0423	0.001	0.0420	0.0483	0.005	0.0449	0.0426	0.001	0.0418
m4	0.0299	0.001	0.0296	0.0325	0.002	0.0293	0.0312	0.001	0.0291
dilution:			0.3450			0.2080			0.2920
χ			0.1760			4.7400			5.7500

The distribution was measured after 48 hours of incubation was assessed in Jurkat cells without drugs (control) or treated with either 0.5 μg × mL^-1 of edelfosine that caused less than 1% of apoptosis (0-1%) or with 1.0 μg × mL^-1 of edelfosine that caused 4-5% of apoptotis (4-5%). Best fit obtained with Isodyn is depicted in column "fit". For Ribose, dilution (initial fraction with respect to the newly synthesized fraction during the treatment) is also shown

ISSN: 1752-0509