Figure 6

E2-dependent transcription is increased in 3T3T cells stably transfected with a P2P-R antisense oligonucleotide. Native 3T3T, and 3T3T cells that have been stably transfected with a P2P-R antisense oligonucleotide (AS), to knockdown P2P-R protein expression, were transfected with ERE-luciferase plasmid (300 ng/well) plus renilla luciferase plasmid (15 ng/well) and pSG5-ERα (50 ng/well) and then incubated for 18-24 h in phenol-red free DMEM with 5% CS-FBS in the presence of DMSO or 100 pM 17 β-estradiol. RLA, relative luciferase activity (RLA represents the firefly luciferase RLU divided by the renilla luciferase RLU for the same sample). Experiments were performed in triplicate, bars denote standard error.