Figure 1

Commonly significantly regulated transcripts by TNT in vitro and in vivo. For the in vivo experiment, rats were exposed to TNT at various doses: 0 (control), 4.8, 48, 96 or 192 mg/kg for 24 h or 48 h. Subsequently rats were sacrificed and RNAs were isolated for microarray hybridization using a Rat Agilent whole genome array. For in vitro experiments, primary cultured rat liver cells were treated with TNT at 10 mg/l or vehicle control, and RNAs were isolated for microarray experiments using the same type of Agilent array as the in vivo experiment. Differentiated transcripts were analyzed as described in the Materials and Methods. The commonly regulated transcripts are shown in the intersection part of the Venn diagram.