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Table 4 Comparison of selected fluxes across different growth conditions.

From: Reconstruction and analysis of genome-scale metabolic model of a photosynthetic bacterium

Reaction
name
Autotrophy Minimum flux Maximum flux Mixotrophy Minimum flux Maximum flux Dark Heterotrophy Minimum flux Maximum flux Light Heterotrophy Minimum flux Maximum flux Reaction description
2.7.1.2a 0 0 0 0.567 0.566 0.567 0.567 0.566 0.567 0.567 0.566 0.567 beta-D-glucose + ATP → beta-D-glucose-6-phosphate + ADP
4.2.1.2 12.67 12.667 +∞ 14.67 14.657 +∞ 0.905 0.884 +∞ 2.148 1.836 +∞ malate ↔ fumarate + H2O
5.3.1.6 1.201 1.2 +∞ 1.269 1.269 +∞ -0.054 -0.051 -0.055 0.066 0.067 +∞ D-ribose-5-phosphate ↔ D-ribulose-5-phosphate
_UQ 0.8 0 0.8 0.8 0 0.8 0 0 0 0.8 0 0.8 PSII* + UQ + 2 H+ → PSII + UQH2
_1.6.5.3 0 0 +∞ 0 0 +∞ 2.134 0 +∞ 0 0 +∞ NADH + UQ + 7 H+ → NAD+ + UQH2 + 4 H+_peribac
_3.6.3.14 38.348 15.7 +∞ 21.727 21.7 +∞ 4.98 4.95 +∞ 6.292 6.281 +∞ 3 H+_peribac + phosphate O4P + ADP ↔ 3 H+ + H2O + ATP
6.2.1.1 0.008 -∞ +∞ -30.017 -∞ +∞ -2.124 -∞ +∞ -4.635 -∞ +∞ coenzyme A + acetate + ATP ↔ acetyl-CoA + diphosphate + AMP
  1. Units in mmol gDW-1 h-1. 2.7.1.2a, glucokinase, is the reaction that phosphorylates beta-D-glucose upon entrance in the cell, marking the start of the glycolysis. The flux direction changes can be seen in reaction 4.2.1.2, fumarate hydratase, from TCA cycle and 5.3.1.6, ribose-5-phosphate isomerase, from the pentose phosphate pathway. _UQ and _1.6.5.3 are reactions that reduce UQH2 from photosystem II or NADH oxidation, respectively, causing a pumping of protons to the thylakoid. _3.6.3.14 is the ATP synthase that forms ATP shuttling protons from the thylakoid to the cytosol. 6.2.1.1, acetate-CoA ligase, is the reaction that generates acetyl-CoA from acetate and coenzyme A, that would be a major flux hub in an ethanol-producing strain, standing as the first step of fermentation.