Figure 3

Extracting the BCR-dependent network that regulates the cellular response. The Schematic of the analysis performed to identify the core regulatory network inducing G1 arrest upon anti-IgM stimulation by combining our experimental data and publicly available PPI databases using network analysis is shown in panel A. Panel B depicts the dense overlapping regulatory module identified for TFs regulating the early induced genes due to BCR receptor stimulus. This was achieved by applying TFBS prediction algorithm and literature curation. The red nodes are the early genes induced genes. The yellow nodes represent TFs activated while the blue nodes represent the TFs repressed either at 20 or 40 minutes. Panel C shows the network emanating from BCR that induces changes in the activity of key signaling intermediates analyzed (green nodes) leading to either activation or repression of the set of transcription factors identified from TF array (The nodes are colour coded as in panel B). The nodes in light blue and grey represent other signaling and transcriptional intermediates identified from the network analysis. The set of genes affected due to anti-IgM stimulation are represented as red nodes. All the intermediate nodes are labeled to human orthologs with their corresponding Entrez Gene ID.