Figure 4

Identifying the key molecular intermediates involved in mediating BCR-dependent cell cycle arrest. Panel A shows the population of G1 arrested cells under RNAi mediated knockdown of early induced genes identified as immature B cell specific. The plot represents mean of three individual replicates with ± S.D. Panel B lists chemical inhibitors screened for protection of CH1 cells from anti-IgM mediated G1 arrest to identify potential regulatory signaling intermediates. Panel C shows the histograms of live population of cells in each of the cell cycle phases under normal anti-IgM stimulation, in presence of p38 inhibitor SB203580 and CaMKII inhibitor KN62. The decrease in the population of G1 arrested cells in both the cases of SB203580 and KN62 could be clearly observed in the histograms. Effect of SB203580 and KN62 on early induced genes one hour after anti-IgM stimulation is shown in panel D. The plot indicates mean value of the fold change in the mRNA levels of the respective genes in log scale obtained from three replicates with ± S.D.