Constructs | N-degron | C-degron | Bidirectional degron | Source |
|---|
TDegF |
+
|
-
|
-
| [25] |
TDegM |
-
|
-
|
-
| [25] |
GFP-cODC1-TDegF-RFP |
+
|
+
|
+
| This work |
GFP-cODC1-TDegF |
+
|
+
|
+
| This work |
cODC1-TDegM |
-
|
+
|
-
| This work |
cODC2-TDegF |
+
|
+
|
+
| This work |
- The sequence of TDegM is similar to TDegF with the exception of the TEV protease cleavage site (ENLYFQ-M replaces ENLYFQ-F). The sequences for cODC1-TDegF and cODC2-TDegF differ in the length of the cODC sequence inserted into TDegF (Figure 1A, C). The ability to act as an N-degron, a C-degron or a bidirectional degron is indicated for all constructs (+).
