|
Constructs
|
N-degron
|
C-degron
|
Bidirectional degron
|
Source
|
|---|
|
TDegF
|
+
|
-
|
-
|
[25]
|
|
TDegM
|
-
|
-
|
-
|
[25]
|
|
GFP-cODC1-TDegF-RFP
|
+
|
+
|
+
|
This work
|
|
GFP-cODC1-TDegF
|
+
|
+
|
+
|
This work
|
|
cODC1-TDegM
|
-
|
+
|
-
|
This work
|
|
cODC2-TDegF
|
+
|
+
|
+
|
This work
|
- The sequence of TDegM is similar to TDegF with the exception of the TEV protease cleavage site (ENLYFQ-M replaces ENLYFQ-F). The sequences for cODC1-TDegF and cODC2-TDegF differ in the length of the cODC sequence inserted into TDegF (Figure 1A, C). The ability to act as an N-degron, a C-degron or a bidirectional degron is indicated for all constructs (+).
