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Table 1 Single Substrate Assay: Compound IC50 Values (uM)

From: Two additive mechanisms impair the differentiation of 'substrate-selective' p38 inhibitors from classical p38 inhibitors in vitro

Compound phospho-MK2 phospho-ATF2 substrate selectivity*
SD-0006 0.11 ± 1.2 0.35 ± 1.1 3.3
BIRB 796 7.75 ± 1.3 0.92 ± 1.1 0.12
CMPD1 23.4 ± 1.9 >400** >17
  1. IC50's are shown ± standard error.
  2. *Substrate Selectivity is defined as a relative measure between inhibiting phospho-ATF2 and phospho-MK2 = (ATF2 IC50)/(MK2 IC50). This quantity is distinct and separable from a compound's potency.
  3. **CMPD1 is unable to completely inhibit phospho-ATF2 within its solubility limits.