Two additive mechanisms impair the differentiation of 'substrate-selective' p38 inhibitors from classical p38 inhibitors in vitro

Table 2 Biochemical Kinetic Model Parameters

Name	Description	Value	Units	Reference
K _{D, ATP}	ATP-p38 affinity	67	uM	In-house measurement
K _{D, ATF2}	ATF2-p38 affinity	38	uM	[30]
K _{D, MK2}	MK2-p38 affinity	20	nM	[31]
k _{cat, ATF2}	p38-ATF2 catalytic rate	1.2	s^-1	[30]
k _{cat, MK2}	p38-MK2 catalytic rate	0.17 2.4	s^-1 s^-1	[31] In-house measurement
[p38]	p38 (active) initial condition	0.5	nM
[ATP]	ATP initial condition	50	uM
[ATF2]	ATF2 initial condition	100	nM
[MK2]	MK2 initial condition	10	nM

ISSN: 1752-0509