Skip to main content

Table 2 Possible new annotations for C. thermocellum ORFs based on identified metabolic gaps

From: Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production

Missing EC number Enzyme name Possible Cth ORF Current annotation Reciprocal best hita E valueb diacylglycerol kinase Cthe_3168 hypothetical protein A0R923 7.00E-35 Hexosaminidase Cthe_0787 isoleucyl-tRNA synthetase A4N847 0 CDP-glycerol pyrophosphorylase Cthe_1276 pantetheine-phosphate adenylyltransferase A1SHB9 3.00E-07 phosphatidylserine synthase Cthe_0158 Ribonuclease A9JBA9 1.00E-68 phosphatidylserine decarboxylase Cthe_0505 formate acetyltransferase A4NBN7 0 succinate dehydrogenase Cthe_2355 L-aspartate oxidase Q97W79 1.00E-94 NADH-fumarate reductase Cthe_2355 L-aspartate oxidase B0VG44 5.00E-98 succinate dehydrogenase Cthe_2355 L-aspartate oxidase A4YEK0 2.00E-85 succinate--CoA ligase Cthe_1907 amino acid adenylation domain A3P3B3 3.00E-40 acetyl-CoA carboxylase Cthe_0699 carboxyl transferase A0RY61 8.00E-169 biotin carboxylase Cthe_0949 carbamoyl-phosphate synthase, large subunit B2J980 0 aminodeoxychorismate lyase Cthe_0026 queuosine biosynthesis protein Q03L66 3.00E-54 alkaline phosphatase Cthe_2965 binding-protein-dependent transport systems inner membrane component B0USD4 1.00E-59 alanine transaminase Cthe_0755 aminotransferase, class I and II Q7LYW0 3.00E-66 serine--pyruvate transaminase Cthe_0265 aminotransferase, class V B4BE13 0 homoserine kinase Cthe_0397 ABC transporter related protein A5 M0U7 3.00E-140 phosphoserine phosphatase Cthe_0256 histidine kinase A9G173 3.00E-54 pyruvate kinase Cthe_1955 RNA binding S1 A5LC67 0 formate dehydrogenase Cthe_0199 4Fe-4S ferredoxin, iron-sulfur binding Q2LVY6 9.00E-11 nitrate reductase Cthe_0200 FAD-dependent pyridine nucleotide-disulphide oxidoreductase Q11VH4 4.00E-24 transaldolase Cthe_0217 Glucose-6-phosphate isomerase Q2S6E8 1.00E-22 GMP synthase Cthe_0375 GMP synthase, large subunit A2C5P2 4.00E-176 formate dehydrogenase Cthe_0341 NADH dehydrogenase (quinone) B5IPC7 6.00E-126 isopentenyl-diphosphate Delta-isomerase Cthe_1022 Glycerol-3-phosphate dehydrogenase A8VXT5 2.00E-94 farnesyltranstransferase Cthe_0831 Polyprenyl synthetase B2J443 4.00E-69 trans-pentaprenyltranstransferase Cthe_0564 Trans-hexaprenyltranstransferase Q6KZR8 3.00E-25 lactase Cthe_0212 Beta-glucosidase P09848 3.00E-89 nicotinamidase Cthe_1178 isochorismatase hydrolase Q6F6U3 6.00E-08 branched chain keto acid dehydrogenase Cthe_0547 periplasmic solute binding protein A8VXE7 2.00E-27
  1. aUniProt accession numbers
  2. bE value based on reciprocal best hit against C. thermocellum gene