Skip to main content

Table 2 Gene deletion strategies for ethanol production in E. coli

From: OptORF: Optimal metabolic and regulatory perturbations for metabolic engineering of microbial strains

Deleted Genes Growth Rate (hr-1) Ethanol Yield (%)
none (wild-type) 0.467 39.3
arcA pgi     0.122 83.5
nuoN pgi     0.121 83.6
fnr gntR pflB tdcE pgi 0.225 86.2
arcA gntR pta eutD tpiA 0.244 89.3
fnr gntR pflB tdcE tpiA 0.235 90.5
  1. The predicted ethanol yields and growth rates are listed for two-deletion strategies and five-deletion strategies. Three or four deletion strategies are not shown because the ethanol yield was not significantly improved over two deletion strategies. The variability in ethanol yield at the predicted maximum growth rate was zero or very small (< 0.01%) for all cases. The ethanol yield is reported as % of the maximum theoretical yield (100% is 0.51 g ethanol/g glucose or 2 mol ethanol/mol glucose). Maximum glucose uptake rate of 18.5 mmol/gDW/hr was used to simulate anaerobic growth conditions.