Deleted Genes | Overexpressed Genes | Growth Rate (hr-1) | Isobutanol Yield (%) |
|---|
adhE
|
gdhA
| Â | Â | 0.223 | 89.5 |
adhE
|
gntR
|
pgi
| Â | 0.128 | 93.8 |
adhE
|
pgi
|  | edd       fbp | 0.128 | 94.3 |
adhE
|
pntA
|
nuoN
| edd       fbp | 0.110 | 95.1 |
adhE
|
pntA
|
gdhA
| edd       fbp | 0.102 | 95.5 |
- The predicted isobutanol yields and growth rates are listed for gene deletion and overexpression strategies. The two enzymes needed for synthesis of isobutanol (KDC and ADH) were assumed to be present for all cases. The variability in isobutanol yield at the predicted maximum growth rate was zero or very small (< 0.01%) for all cases. The isobutanol yield is reported as % of the maximum theoretical yield (100% is 0.41 g isobutanol/g glucose or 1 mol isobutanol/mol glucose). The simulation conditions were the same as the ethanol case.
