From: Spatio-temporal modeling of signaling protein recruitment to EGFR
Binding domain | Target | Kd | Kon | Koff | Method | Reference |
---|---|---|---|---|---|---|
SHC PTB | EGFR (32 PY peptides) | <2 μM |  |  | Protein microarray & SPRa | [26] |
 | EGFR PY1148 peptide | 28 nM |  |  | ITCb | [71] |
 | TRK PY490 peptide | 42 nM |  |  |  |  |
SHC SH2 | EGFR PY1148 peptide | NBc | Â | Â | SPR | [27] |
 | EGFR PY1173 peptide | 65 nM |  |  |  |  |
PLCγ1 SH2 | EGFR PY peptides | <2 μM |  |  | Protein microarray | [26] |
PLCγ1 N+C SH2 | EGFR PY992 | 200 nM |  |  | SPR | [28] |
 | EGFR PY1173 | 740 nM |  |  |  |  |
STAT1,3 SH2 | EGFR PY peptides | >2 μM |  |  | Protein microarray | [26] |
Grb2 SH2 | EGFR PY1068 peptide | 30 nM | Â | Â | SPR | [27] |
 | EGFR PY1086 peptide | 60 nM |  |  |  |  |
 | EGFRPY1148 peptide | NB |  |  |  |  |
 | EGFR PY1173-7Y4 | NB |  |  |  |  |
 | EGFR PY992-8Y4 | NB |  |  |  |  |
 | EGFR phosphopeptides | <2 μM |  |  | Protein microarray | [26] |
Grb2 protein | EGFR PY1068 peptide | 380 nM | Â | Â | ITC, SPR | [31] |
 | EGFR PY1068 peptide | 713 nM |  |  | SPR | [29] |
 | Activated EGFR in A431 membranes (fractional) | 97-650 nM | 0.016/nM/s (78%) | 7.5-8.1/s (89%) | Single molecule analysis | [32] |
 |  |  | 0.005/nM/s (21%) | 1.6-2.6/s (<11%) |  |  |
 |  |  | 0.0022/nM/s (1%) | 0.07-0.4/s (<5%) |  |  |
p85 N-SH2 | PDGFR PY315 peptide | 300 nM | Â | Â | ITC, SPR | [72] |
p85 2 SH2 | IRS-1 PY | 0.3-3.0 nM | 0.03-0.4/nM/s | 0.11-0.19/s | SPR | [73] |
Src SH2 | (YMXM) peptides polyoma middle tumor antigen | 600 nM | Â | Â | ITC | [74] |
Lck SH2 | Lck Y505 peptide | 4 μM |  |  | ITC, SPR | [72] |
 | EGFR C-terminus | 5 μM |  |  | ITC | [74] |