The predicted secondary structures and estimated properties of high-and low-expressing synthetic RDS sequences. The RDS nucleotides considered in this calculation are shown in upper-case letters, and the estimated translational efficiencies (E), measured relative expression levels (L
), overall RDS exposure probabilities (P
), probability of ribosome-bound mRNA (P
), RRS-anti RRS hybridization energies (ΔG
) and the Gibbs free energies (ΔG) of the helix structure inside RDS are also shown for sRDS11 (A), sRDS2 (B), sRDS22 (C) and sRDS9 (D). The secondary structures were predicted by UNAFold.