Figure 7

Effects of CREB motif count on gene expression in the subspace of the conserved eigensystem 3 (A, B) The effects of CREB motif count per gene on the average loadings of the eigensystem A3 or M3 analyzed by weighted linear regression, as described in the legend to Figure 6. (C, D) Effect of CREB count and direct and indirect (via CREB count) effect of CNS count per gene on the average loadings of eigensystems A3 or M3 analyzed by weighted linear regression, either univariate (edges 1, 2, 3) or bivariate (edges 4, 5), in groups of genes with the same numbers of CNSs, CREB motifs, or both. The results are represented as path analysis graphs, with each edge marked by the values of the respective linear regression directional coefficient α and its corresponding t-test p-value. In the univariate regression of CREB count on CNS count (edge 3) the data for all the genes with at least one CNS in the TRAM database were used. (E) The single gene A3 loadings and CREB counts for all the genes with CNS(s) in the kainate dataset (grey dots) compared to the values the Creb-binding genes in PC12 cells identified by genome-wide ChIP analysis by Impey et al. [50] (blue dots). (F) Uncorrelated, additive effects of the motifs SATB and CREB on gene log-expression provide an insight into the biology of the MCAO system.