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Table 4 Growth phenotypes predicted by the model under a variety of routinely utilized laboratory conditions*

From: iRsp1095: A genome-scale reconstruction of the Rhodobacter sphaeroides metabolic network

  Light Dark
   Electron Acceptor
   O2a DMSOa None
Succinate + NH3 +/+b +/- +/- -/-
Succinate + Glutamate +/+ b +/- +/- -/-
Lactate + NH3 +/+ b +/- +/- -/-
Glutamate only +/- +/- +/- -/-
CO2 + H2 + NH3 +/- -/- -/- -/-
CO2 + H2 + N2 +/- -/- -/- -/-
  1. * +/+ Growth and H2 production predicted; +/- Growth but no H2 production predicted; -/- No growth
  2. aOxygen (O2) or DMSO was used as the sole electron acceptors in simulations.
  3. b Succinate and lactate uptake rates were set to 3 mmol/g DW h, while the NH3 and glutamate uptake rates were set to 1 mmol/g DW h, as these are within the rate of experimentally observed uptake rates for these substrates. CO2, H2 and N2 uptake rates were set to 1 mmol/g DW h for photoautotrophic growth simulations.